Monoclonal antibody sequencing
Monoclonal antibody sequencing is the first step to antibody engineering: optimization and recombinant expression. This experiment allows for a fast, targeted sequencing of the DNA sequence expressing the antibody. In the case the hybridoma cell line has been lost, and if the antibody is available in sufficient amount, it is possible to identify the antibody sequence by mass spectrometry.
• Hybridoma expressing the antibodies to sequence
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