Mechanism of action of antibacterial compounds
It may be necessary to know the mechanism by which an antibiotic exerts its antibacterial action. A set of techniques can be used to identify the mechanism of action of antibiotics: study of the permeabilizing action of the plasma membrane, microscopy and comparative observation of morphotypes caused by control and tested compounds, and radiolabelling techniques to measure the incorporation of precursors in the presence of control antibiotics.
• Compounds to test
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