Microbial identification by DNA sequencing
One of the most accurate ways of determining the taxonomical group of microorganisms is through the sequencing of very specific regions in their genome. A PCR is conducted for amplifying these highly variable regions - RNA 16S in bacteria; RNA 5.8S gene and ITS1, ITS2 regions in fungi-, using primers that are homologous to very conserved regions. This allows the assignment of the specimen to the closest taxonomical group by comparing the sequence obtained with available databases.
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