Plasmid site-directed mutagenesis
Site-directed mutagenesis is a set of techniques that allows the specific swapping, the deletion or the addition of one or a few nucleotides in a double stranded DNA sequence. It requires the usage of a PCR amplification of the sequence using primers containing the alteration of the sequence. Amplified fragment can be reintegrated into a new vector, or the entire vector can be sequenced for verification of integrity.
• Original sequence to mutate in expression vector
• Map of the origin plasmid
• Sequence/accession number of the gene to mutate
• Description of available primers you can provide
• Detailed description of the mutations you require
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