Genomics Cloning Plasmidic expression vector cloning
Molecular cloning is a process that allows the construction of recombinant DNA for the purpose of expressing genes in a given organism. The steps of cloning are typically: obtaining a source containing the DNA fragment of interest, amplification by PCR, insertion into a DNA vector. The set is inserted into a primary host for selection and replication (i.e. E. coli), and can then be purified to be introduced into a secondary host.
• Source of the gene and information • Primers and their sequences • Empty plasmid and its map Further details on the project may be requested directly by the expert. You will be able to exchange directly with the experts after filling in the form below.
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