Plasmidic expression vector cloning
Molecular cloning is a process that allows the construction of recombinant DNA for the purpose of expressing genes in a given organism. The steps of cloning are typically: obtaining a source containing the DNA fragment of interest, amplification by PCR, insertion into a DNA vector. The set is inserted into a primary host for selection and replication (i.e. E. coli), and can then be purified to be introduced into a secondary host.
• Source of the gene and information
• Primers and their sequences
• Empty plasmid and its map
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