Digital PCR - dPCR & ddPCR
Digital PCR (dPCR or ddPCR) is a technique that separates the reaction of amplification of a specific DNA target by PCR into a large number of events. Each event is measured for positive/negative amplification, and the number of positive amplifications will provide the absolute quantification of initial specific DNA target in each sample using statistical laws.
• Samples in sufficient amount
• Primers and probes, or accession number of the gene of interest
• Information regarding requested amplifications
• Description of the samples and QC data
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