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CRISPR/Cas9: Vector preparation and cell line edition

CRISPR/Cas9 is a powerful tool for genome editing. It requires the construction of a DNA vector that codes both for Cas9 protein and a target specific RNA called crRNA (also called single-guide...

Mammalian cell lines

CRISPR/Cas9 is a powerful tool for genome editing. Using a specific vector, cell lines to be modified are transfected or infected by viral systems for DNA delivery. Edited cell lines are...

Plants

The CRISPR/Cas9 method is a powerful tool for genome editing that can be useful for agriculture and agri-food research. It requires the construction of a DNA vector that codes for both the...

Microorganisms

Microorganism genome editing generates stable clones using homologous recombination at the loci of the genes to edit. This genetic engineering can add (knock-in), remove (knock-out) or modify...