CRISPR/Cas9: Vector preparation and cell line edition
CRISPR/Cas9 is a powerful tool for genome editing. It requires the construction of a DNA vector that codes both for Cas9 protein and a target specific RNA called crRNA (also called single-guide RNA when combined with another sequence, tracrRNA) that must bind only where editing is desirable. In this experiment, the Expert will design and construct the DNA vector that should allow for the edition of the sequence in the target cell's DNA.
• Accession number of genes of interest
• BLAST data if specificity data available
• Cell lines to transfect
• Antibodies and conditions
Further details on the project may be requested directly by the expert. You will be able to exchange directly with the experts after filling in the form below.
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