Mammalian cell lines
CRISPR/Cas9 is a powerful tool for genome editing. Using a specific vector, cell lines to be modified are transfected or infected by viral systems for DNA delivery. Edited cell lines are selected, and the modifications of the genome are sequenced for verification. Monoallelic vs biallelic edition can be chosen, as well as an optional removal of the selection cassette after integration.
• Accession number of genes of interest
• Guide vector preparation and information
• Cell line
• Antibodies and conditions
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