Phosphoproteomics looks at the phosphorylation of proteins as a post-translational modification in a set of samples by mass spectrometry. Phosphorylation is a key modification that can be reversible, and that is involved in many cellular functions and pathways. Phosphoproteomics covers two main goals: understanding which protein or pathway is activated in a sample, and determining which proteins might be a potential candidate for a drug target.
• Samples to analyze in sufficient amount
• Description of your samples
• Specific requirements description (if immunoprecipitation or chromatography enrichment)
• Size of the proteins of interest
• Picture of the gel
• Description of buffers
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