In vitro assays
Apoptosis corresponds to the genetically-controlled self-destruction of individual cells not leading to an inflammatory response. Multiple techniques allows for the measurement of apoptosis within a cell population, including measurement of caspases activity, cytochrome C release, nuclear staining, flow cytometry based techniques such as TUNEL, mitochondrial transmembrane potential measurement and protein markers measurement.
• Cell cultures with growth conditions
• Vectors to transfect and conditions
• Compounds to add and conditions
• Additional treatments conditions
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