Immortalized cell lines development

Cell culture Lines Immortalized cell lines development

Service description

This service includes the preparation of immortalized cell lines from purified cells, for exemple primary cultures. This involves culturing the cells, immortalizing them, and culturing them to verify the immortalization rate. Immortalized cells can then be shipped.

Materials to provide

Minimum 2 million cells
Minimum 1l of basal medium and flasks if coated
Further details on the project may be requested directly by the expert. You will be able to exchange directly with the experts after filling in the form below.

Delivrable(s)

  • Cells culturing
  • Immortalization
  • Determination of immortalization efficiency (> 30 passages minimum confirmation)
  • Mycoplasma test
  • Controls according to the immortalization method (qPCR or eq)
  • Images of cells
  • Critical reagent test if required
  • 3 x 1ml frozen vials included with minimum 500,000 cells in each
  • Study following the specifications validated with the Expert

Your project

Please answer all questions below to specify your requirements