Stable cell lines development
Stable cell line generation is a set of technique that allows for the long-term expression or inhibition of a gene of interest in an eukaryotic cell. Culture cells are transfected by linear or circular DNA and plated. Cells are then isolated into separated wells, selected for the presence of the transfected DNA (typically using antibiotics) and analyzed for the expression of the gene of interest.
• Cell lines to stably transfect and culture conditions
• Secound round selection marker
• Gene and protein accession numbers if necessary
• Delivery format
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