Stable cell lines development

Cell culture Lines Stable cell lines development

Service description

Stable cell line generation is a set of technique that allows for the long-term expression or inhibition of a gene of interest in an eukaryotic cell. Culture cells are transfected by linear or circular DNA and plated. Cells are then isolated into separated wells, selected for the presence of the transfected DNA (typically using antibiotics) and analyzed for the expression of the gene of interest.

Materials to provide

• Cell lines to stably transfect and culture conditions
• Secound round selection marker
• Gene and protein accession numbers if necessary
• Delivery format
Further details on the project may be requested directly by the expert. You will be able to exchange directly with the experts after filling in the form below.

Delivrable(s)

  • Stable clones generation
  • 3 clones included per sample
  • Verification by qPCR and/or Western blot
  • Clones delivered in 3 x 1ml frozen vials per clone or in agreed format
  • Culture conditions
  • Study following the specifications validated with the Expert

Your project

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