Chip methylation analysis

Genomics Microarray Chip methylation analysis

Service description

The aim of this service is to measure, after conversion to bisulfite, the methylation levels of CpG sites distributed throughout the genome by quantitative genotyping using the Illumina Infinium Methylation EPIC chip technique (850K). The number of samples analyzed in parallel on the chip is 8 samples with possible conditioning of 16, 32 or 96 samples. This analysis can be performed using genomic DNAs or DNAs from paraffin blocks (FFPE).

Materials to provide

1000 ng of genomic DNA or 2000 ng of DNA from FFPE tissues
The size of the fragments must be greater than or equal to 2 Kb (photo of agarose gel or TapeStation profile)
If possible in a volume of 20 µL
Elution Buffer Information
96-well plate preparation with associated plate plane
No biohazard samples

Delivrable(s)

  • Raw intensity files (.idat format)
  • Final report of methylated and unmethylated cytosine ratios (.txt format)
  • Project report in GenomeStudio (.bsc format)
  • Sample sheet corresponding to the repair of samples on chips (.csv format)
  • Chip decoding file (.dmap format)
  • On-chip methylation probe distribution manifest (.bmp format)
  • QC file corresponding to the % of CpG probes detected with a P-value of 1% and 5%.

Your project

Please answer all questions below to specify your requirements