Recombinant proteins production in bacteria

Bioprocess Proteins Recombinant proteins production in bacteria

Service description

Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cells. Proteins can be recombinant, meaning they are generated from engineered DNA expression vector, that allows for the addition of a tag and higher expression level. In this experiment, the expression is made in a bacterial cell model, which allows for a higher yield of protein purified at a lower cost.

Materials to provide

• Accession number of protein of interest
• Plasmids or transformed bacteria
• Expression conditions if known
Further details on the project may be requested directly by the expert. You will be able to exchange directly with the experts after filling in the form below.

Delivrable(s)

  • Culture/Transformation
  • Purification of proteins
  • Degree of purity
  • Frozen protein in aliquots
  • Gel picture after purification
  • Quantification
  • Full disclosure of the purification process
  • Study following the specifications validated with the Expert

Your project

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