Recombinant proteins production in bacteria
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cells. Proteins can be recombinant, meaning they are generated from engineered DNA expression vector, that allows for the addition of a tag and higher expression level. In this experiment, the expression is made in a bacterial cell model, which allows for a higher yield of protein purified at a lower cost.
• Accession number of protein of interest
• Plasmids or transformed bacteria
• Expression conditions if known
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