Recombinant proteins production in insect cells
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cell lysates. Proteins can be recombinant, meaning they are generated from engineered DNA expression vector, that allows for the addition of a tag and higher expression level. In this experiment, the expression is made in a insect cell model, which typically allows for post-translational modifications and in some cases a better folding of the protein.
• Accession number of protein of interest
• Bacmid or low-titer viral stock
• Expression conditions if known
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