Recombinant proteins production in insect cells

Bioprocess Proteins Recombinant proteins production in insect cells

Service description

Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cell lysates. Proteins can be recombinant, meaning they are generated from engineered DNA expression vector, that allows for the addition of a tag and higher expression level. In this experiment, the expression is made in a insect cell model, which typically allows for post-translational modifications and in some cases a better folding of the protein.

Materials to provide

• Accession number of protein of interest
• Bacmid or low-titer viral stock
• Expression conditions if known
Further details on the project may be requested directly by the expert. You will be able to exchange directly with the experts after filling in the form below.

Delivrable(s)

  • Culture/Infection/Transfection
  • Purification of proteins
  • Degree of purity
  • Frozen protein in aliquots
  • Gel picture
  • Quantification
  • Full disclosure of the purification process
  • Study following the specifications validated with the Expert

Your project

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