Recombinant proteins production in mammalian cells

Bioprocess Proteins Recombinant proteins production in mammalian cells

Service description

Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cells. Proteins can be recombinant, meaning they are generated from engineered DNA expression vector, that allows for the addition of a tag and higher expression level. In this experiment, the expression is made in a mammalian cell model, which allows for extensive post-translational modifications and appropriate folding of the expressed protein.

Materials to provide

• Accession number of protein of interest
• Expression vector and its map
• Purification conditions if known
• Cell lines and growth conditions if stable cell line selected
Further details on the project may be requested directly by the expert. You will be able to exchange directly with the experts after filling in the form below.

Delivrable(s)

  • Culture/Infection/Transfection
  • Expression and purification of proteins
  • Degree of purity as requested
  • Frozen protein in aliquots
  • Gel picture after purification
  • Quantification
  • Full disclosure of the purification process
  • Study following the specifications validated with the Expert

Your project

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