Recombinant proteins production in mammalian cells
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, typically cells. Proteins can be recombinant, meaning they are generated from engineered DNA expression vector, that allows for the addition of a tag and higher expression level. In this experiment, the expression is made in a mammalian cell model, which allows for extensive post-translational modifications and appropriate folding of the expressed protein.
• Accession number of protein of interest
• Expression vector and its map
• Purification conditions if known
• Cell lines and growth conditions if stable cell line selected
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