2D electrophoresis allows the separation and quantification of whole proteins, in contrast to bottom-up LC-MS/MS approaches in which proteins are digested into peptides. This separative method can be used to visualize the profile of a moderately complex protein extract or to detect different isoforms of the same protein (post-translational modifications). A qualitative or quantitative analysis can be performed with the analysis of gel images using dedicated software.
Protein extracts in a buffer adapted to the 2D-DIGE approach
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