ELISA: Assay development

Proteomics Quantification ELISA: Assay development

Service description

In the case an ELISA test is routinely used, it might be interesting for a lab to develop its own. In this experiment, an expert can develop ELISA kits according to specific needs in terms of strategy, detection method, and quantity needed.

Materials to provide

• Primary and secondary antibodies
• Antigen with QC data
• Competitive substrate
• Positive and negative controls, or accession number of proteins of interest
Further details on the project may be requested directly by the expert. You will be able to exchange directly with the experts after filling in the form below.

Delivrable(s)

  • Development and optimization of the test
  • Labeling of primary antibody (direct approach) or secondary antibody (sandwich approach), or adding sufficient amount of labelled secondary antibody (competitive approach)
  • Full detailed protocol
  • Preparation of the plates
  • Study following the specifications validated with the Expert

Your project

Please answer all questions below to specify your requirements