Western blot is a very popular method to determine the amount of a target protein in a sample. It is based on the sensitive recognition of an epitope by a specific antibody, followed by the recognition of the primary antibody by a secondary antibody typically coupled to a detection compound such as a fluorophore or a chemiluminescence substrate.
• Samples in sufficient amount
• Description of the samples, amount, buffers, quantitation data
• Information regarding how the samples must be loaded (amount, order etc.)
• Antibodies or accession numbers of the proteins of interest and references
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