Ribosome profiling or Ribo-seq is a technique that allows for the determination of actively translated mRNA in a sample. Using an initial treatment of ribonuclease followed by a protease treatment and sequencing, Ribo-seq provides the exact location of ribosomes on mRNA, thus enabling the identification of translation start sites, the complement of translated ORFs in a cell or tissue, the distribution of ribosomes on a mRNA, and the speed of translation.
• Proof of samples quality or integrity (Bioanalyzer traces or eq.)
• Rnase treatment information
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