Molecule-lipid interaction measurement by Langmuir monolayers
The interaction of molecules with lipids can be measured by the monolayer technique at the water-air interface. Applications: identification of receptors for viruses or bacteria, identification of lipids recognized by pore-forming antibiotics, evaluation of the interaction of amyloid proteins with cellular lipids,...
In this technique, lipids are spread on the surface of a drop of aqueous solution at the water-air interface (hydrophobic/lipophilic/apolar part towards air, hydrophilic/polar part towards water) forming a monolayer. Molecules to be tested are then injected into the aqueous phase and their interaction with the lipid film is monitored by measuring the surface pressure/tension.
• Compounds to test
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