Genome and Exome Sequencing

 

Sanger sequencing -Next generation sequencing

 

Labtoo offers a selection of sequencing services: RNA-Seq, Sanger Sequencing, WES, WGS, New Generation Sequencing (NGS),...

DNA sequencing involves determining the order of nucleotide bases in order to know the sequence of the corresponding protein, or to understand the function of this DNA fragment. There are several sequencing techniques and it is possible to sequence, in addition to DNA, RNA (messenger, non-coding,...), transcriptome,...

 

 

RNA Sequencing

mRNA sequencing involves using NSG techniques to determine the coding mRNAs present in a sample. Two methods can be used to separate coding RNAs from non-coding RNAs: either by hybridizing poly(A) tails using poly(T) probes, or by deplating non-coding RNA using oligomers complementary to ribosomal RNAs. In addition, it may also be interesting to sequence non-coding RNAs (ncRNAs), ncRNA sequencing focuses mainly on miRNAs (<22 nt), small RNAs (<200 nt), and long non-coding lncRNA RNAs (>200 nt).

The heterogeneity of the transcriptome within a potentially high cell population. The single cell RNA sequencing technique (scRNA-Seq) makes it possible to identify rare cell types within a population, through the use of next-generation sequencing.

You also have the option of sequencing the entire transcriptome using the shotgun sequencing technique (WTSS) or ribosome profiling, or Ribo-seq, which is a technique used to determine which mRNAs are actively translated in a sample.

 

Other sequencing techniques

A well-known technique: Sanger sequencing which allows a clear reading after the first 15-30 bases and up to 800-1000 bases. This technique is applied to short and targeted sequences. However, it is possible to perform the entire sequencing of a genome using the WGS technique. You also have the possibility to sequence the entire exome (WGS or WXS), which allows the sequencing of all genes expressed in a cell population.

Sequencing can be done on genetic material recovered directly from a complex environment, such as digestive systems, soils, oceans, etc.... thus allowing metagenomics to be performed.

Sequencing can also be used to identify DNA-bound protein binding sites using the ChIP sequencing technique.

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