Microscopy techniques

Electron mucroscope


Microscopy is a set of imaging techniques, therefore, there are different types of microscopes. Each microscope has its own properties, which makes it possible to use them in many scientific disciplines. There are 4 main types of microscopy: Optical microscopy, electron microscopy, probe microscopy, local probe microscopy and metalentil microscopy.


Labtoo offers you microscopy services for your experiments.



Electron Microscopy

1. Scanning Electron Microscopy (SEM)

A scanning electron microscope (SEM) is a type of microscope that produces images by scanning a surface with an electron focus. Eligible samples for SEM are typically dehydrated or fixed in the case of cells, and can also be cryogenically fixed for lipid observation.
The observation of back-scattering electrons (BSE) can be selected.


2. Transmission Electron Microscopy (TEM)

A transmission electron microscope (TEM) is a type of microscope in which an electron beam is transmitted through a sample to form an image. The sample is typically a section of tissue or cell. The main advantage of the MET is the resolution of the generated images.
An immunological marker with gold particles can be performed to locate one or more proteins or cellular components in a sample.

Transmission electron microscopic observation


Light and fluorescence microscopy

1. Fluorescence microscopy

Fluorescence microscopy refers to any microscope that uses fluorescence to generate an image.
A simple case uses epifluorescence and a combination of filters and dicroic mirror to generate an image. Images typically have little "background noise", so fluorescence microscopy is a classic method in biology. Experimental conditions (treatment, duration, etc.) can be specified as required.


2. Confocal microscopy

Confocal microscopy is a type of fluorescence microscopy used to improve optical resolution and contrast, compared to the use of epifluorescence. Collecting images is typically slower than for other types of microscopy, however the scanning method is ideal for generating 3D images.
Experimental conditions (treatment, duration, etc.) can be specified as required.



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