High-throughput sequencing techniques have revolutionalized functional genomics. These thechniques are more and more popularized, allowing great progress in research.
What is it exactly?
The term NGS, also known as "high-throughput sequencing" refers to different sequencing technologies, amongst which are:
Illumina sequencing (most used)
These technologies allow the sequencing of DNA or RNA much faster than the famous Sanger technique.
How does it work?
Three steps are shared for these techniques:
Banks are created by random DNA fragmentation followed by linking to small specific sequences.
The bank is amplified by clonal amplification methods and PCR.
The DNA is sequenced using different methods depending on the technology.
For what application?
There are many application possible, here are some :
Because it is an evolving techology, NGS can be used for sequencing non-referenced genomic sequences (de novo sequencing), for re-sequencing referenced genomes to find new genetic variants, for analysing transcipts (RNA-Seq), for identifying point mutations (SNPs), for insertin or deleting DNA fragments etc.