Sequencing, Expression & Edition Services

 

Labtoo is the intelligent european Marketplace for R&D in Life Science.

You can find on Labtoo the best Experts in Sequencing, Expression & Edition coming from Academic and private labs. Labtoo makes it simple to get a pricing for the service, discover new partners, and contract with them, thanks to our integrated smart assistant!

Labtoo references Platforms, laboratories and private or public providers in Sequencing, Expression & Edition.

ORDER A SERVICE IN SEQUENCING, EXPRESSION & EDITION

Genome Editing

CRISPR/Cas9: Vector Preparation and Cell Line Edition

  CRISPR/Cas9 Vector prepartion and cell line edition    

CRISPR/Cas9 is a powerful tool for genome editing. It requires the construction of a DNA vector that codes both for Cas9 protein and a target specific RNA called crRNA (also called single-guide RNA when combined with another sequence, tracrRNA) that must bind only where editing is desirable. In this experiment, the Expert will design and construct the DNA vector that should allow for the edition of the sequence in the target cell's DNA.

Genome Editing - Mammalian Cell Lines

Genome editing - mammalian cell lines    

CRISPR/Cas9 is a powerful tool for genome editing. Using a specific vector, cell lines to be modified are transfected or infected by viral systems for DNA delivery. Edited cell lines are selected, and the modifications of the genome are sequenced for verification. Monoallelic vs biallelic edition can be chosen, as well as an optional removal of the selection cassette after integration.

 

Genome Editing - Animals

Genome editing Animals    

CRISPR/Cas9 is a powerful tool for genome editing. It allows for relatively easy and cheap modification of the genome in entire organisms, typically mouse rat or zebrafish. In this experiment, and using vectors already designed and prepared in advance, you will be able to generate modified animals.

Genome Editing - Plants

Genom editing Plants    

CRISPR/Cas9 is a powerful tool for genome editing. It requires the construction of a DNA vector that codes both for Cas9 protein and a target specific RNA called crRNA (also called single-guide RNA when combined with another sequence, tracrRNA) that must bind only where editing is desirable.
In this experiment, CRISPR/Cas9 edition method is applied to plant biology, and allows for the creation of specific plasmids, optimizatoin of protoplasts, test of the plasmids on protoplasts, integration and regeneration of a modified plant.

Genome Editing - Microorganisms

Genome editing - Microorganisms    

Microorganism genome editing generates stable clones using homologous recombination at the loci of the genes to edit. This genetic engineering can add (knock-in), remove (knock-out) or modify genes, according to the specific needs.

Sequencing

mRNA Sequencing

mRNA Sequencing     mRNA Sequencing uses Next-Generation sequencing methods to look at the coding RNA in a given sample. In order to discard the non-coding RNAs, two strategies can be adopted: either the hybridization of poly(A) tails using poly(T) tails, or the depletion of non-coding RNA by removing oligomers complementary to rRNA.

Single-cell mRNA Sequencing

Single-cell mRNA sequencing Heterogeneity of transcriptome can be quite high in a cell population, thus Single-cell RNA sequencing (scRNA-Seq) combines the isolation of a single cell with the powerful Next-generation sequencing techniques, making the identification of rare cell types within a population possible. scRNA-Seq requires a reverse transcription of RNA after cell isolation, followed by an amplification before sequencing.

Non-coding RNA Sequencing

Non coding RNA sequencing    

Non-coding RNA (ncRNA) is a type of RNA molecule that does not translate into a protein. They are involved in multiple cellular processes, including translation (i.e. rRNA and tRNA), post-transcriptional modifications, DNA replication and gene regulation. ncRNA sequencing focuses primarily on miRNAs (about 22 nt), small RNAs (all RNAs less than 200 nt, including miRNA), and long non-coding RNAs (lncRNA, more than 200 nt).

Whole Transcriptome

Whole transcriptome    

Whole Transcriptome Shotgun Sequencing (WTSS) allows for the detection, the sequence and the quantification of all RNA in a given biological sample. Typical applications of RNA sequencing include analysis of changes in transcriptome (i.e. differences in gene expression), post-transcriptional modifications and detection of mutations. In addition to mRNA, non-coding RNA and short RNA are also sequenced with WTSS.

Custom RNA Seq

Custom RNA Seq    

Next-Gen sequencing can generate a lot of data that might not be useful for every project. To focus on the specific transcripts that matters for a given project, it is possible to create libraries only after enrichment or amplification of a set of transcripts. This is particularly useful in the case of difficult samples (such as paraffin-embedded tissue) or with low input of RNA (10ng of total RNA), if the set of focus transcripts is identified.

Ribosomal Profiling

Ribosomal profiling     Ribosome profiling or Ribo-seq is a technique that allows for the determination of actively translated mRNA in a sample. Using an initial treatment of ribonuclease followed by a protease treatment and sequencing, Ribo-seq provides the exact location of ribosomes on mRNA, thus enabling the identification of translation start sites, the complement of translated ORFs in a cell or tissue, the distribution of ribosomes on a mRNA, and the speed of translation.

Short Sequences (Sanger)

Short sequences (Sanger)     The "classical" sequencing of short and targeted sequences. It provides clear reading after the first 15-30 bases and up to 800-1000 bases. This methods requires the amplification of the targeted sequences using specific dNTPs, followed by a purification step and the reading of the amplicons by a dedicated sequencer.

Whole Exome sequencing

While exome sequencing    

Whole exome sequencing (WES or WXS) is a technique that allows the sequencing of all expressed genes in a genome in a cell population. It requires a initial step of detection of exonic DNA (region of the genome that encodes proteins), followed by a high-throughput DNA sequencing.

Cell Population DNA Sequencing

Cell population DNA Sequencing    

Whole genome sequencing (WGS) is the process of determining the entire DNA sequence of a given organism's genome at a single time. This includes chromosomal and mitochondrial DNA, and chloroplasts, if applicable. You will be able to select in this experiment all or parts of the process: extraction, library preparation, sequencing, raw data collection and data analysis.

 

ChIP Sequencing

ChIP sequencing     ChIP Sequencing combines an immunoprecipitation of chromatin and a DNA sequencing to identify the binding sites of DNA-associated proteins, with different degrees of specificity. The epigenetic information obtained regarding gene expression regulation can then be correlated to phenotypic observations.

Microbiome Sequencing

Microbiome sequencing    

Metagenomics study genetic material (i.e. 16S rDNA) coming from complex samples. Samples are generally filtered, DNA is extracted, a library is produced and clones are sequenced by NGS. Data integration is a critical step that required powerful bioinformatics tools, and allow the determination of the operational taxonomic unit (OTU)

Genes Expression

qPCR using Dye

qPCR using Dye    

Quantitative PCR (qPCR) with dye is a powerful technique that combines the amplification of a specific DNA fragment using PCR with the highly-sensitive detection of fluorescence in the sample using specific dyes. The more amplification of DNA in the tube, the more dye will bind the double-stranded DNA and become fluorescent, thus allowing for a quantitative determination of initial specific DNA in the sample.

qPCR using Probes

qPCR using probes    

Quantitative PCR (qPCR) with probes is a powerful technique that combines the amplification of a specific DNA fragment using PCR with the highly-sensitive detection of fluorescence in the sample using specific probes. In this case, multiple probes can be used during the same amplification. Using different fluorophores will allow for the quantification of different amplifications.

Digital PCR

Digital PCR    

Digital PCR (dPCR or ddPCR) is a technique that separates the reaction of amplification of a specific DNA target by PCR into a large number of events. Each event is measured for positive/negative amplification, and the number of positive amplifications will provide the absolute quantification of initial specific DNA target in each sample using statistical laws.

 

Nucleic Acid Extraction

Nucleic acid extraction    

Extraction and purification of high-quality RNA from samples is the first and critical step when performing molecular techniques such as quantitative PCR, Next-generation sequencing or cDNA library construction. 
DNA extraction service includes the handling of the samples (blood, cells from culture or other) in order to extract their DNA. The extracted DNA can be stored of shipped back according to the request.

VIRAL PRODCTION

Lentivirus & Retrovirus Production

Lentivirus and retrovirus production    

Production and titration of viral particles (types lentivirus or retrovirus) from an expression vector or a previous preparation. The preparations are verified and measured by qPCR and/or FACS.

Adenovirus Production

Adenovirus production     Production and titration of viral particles (recombinant Adenovirus type) from an expression vector or a previous preparation.

Adeno-Associated Virus (AAV) Production

Adeno-Associated Virus (AAV) production    

Production and titration of viral particles (recombinant Adeno-Associated Virus AAV type) from an expression vector or a previous preparation.

Microarray

DNA & RNA Microarray

DNA and RNA microarray    

A microarray is a collection of DNA or RNA spots attached to a solid surface. Each spot is exposed to a cDNA or RNA sample for potential hybridization to the specific target, and hybridization level is measured for each spot by detection of fluorophore or chemiluminescence.

microRNA Microarray

MicroRNA Microarray    

MicroRNA microarray is a specific type of microarray that provide a comprehensive coverage of miRNAs in a sample. It provides information regarding the expression level of target miRNA in a given sample with customizable options.

FIND YOUR EXPERT IN SEQUENCING, EXPRESSION & EDITION

Experts of The Week

       
Franz D     e-Zyvec     Jérôme R
Expert en : Genome Editing - Plants, Digital PCR     Expert en : Multigene Cloning, Sub-cloning, Site-Directed Mutagenesis, Custom Recombinant Vector Preparation, CRISPR/Cas9: Vector Preparation and Cell Line Edit     Expert en : qPCR using Dye, Lentivirus & Retrovirus Production, DNA & RNA Microarray, RNA Extraction, Site-Directed Mutagenesis, Sub-cloning, CRISPR/Cas9: Vector Preparation and Cell Line Edition
Find this Expert here     Find this Expert here     Find this Expert here

Discover our other areas of services

 

Analytical Services

Bioinformatics &
Biostatistics Services

Cell Culture, Sorting
 & Cell Assays Services