10 - Protein Detection-1

Service description

2D electrophoresis allows the separation and quantification of whole proteins, in contrast to bottom-up LC-MS/MS approaches in which proteins are digested into peptides. This separative method can be used to visualize the profile of a moderately complex protein extract or to detect different isoforms of the same protein (post-translational modifications). Qualitative or quantitative analysis can be performed with the analysis of gel images using dedicated software.

Materials to provide

Protein extracts in a buffer adapted to the 2D-DIGE approach
Further details on the project may be requested.

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Typical deliverables

  • List of significant variant spots (Excel format)
  • All images and quantification data can be transmitted on request.

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