CDNA library construction

Service description

The construction of a cDNA library is a set of techniques that allows for the cloning of expressed genes in a given cell population into an identical vector. It starts with the extration of mRNA from a cell population, followed by a Reverse transcriptase PCR and an insertion of amplified sequences into a vector. The vector will determine the subsequent usage of the library, i.e. standard library, substractive, full length or Yeast 2-hybrid.

Materials to provide

• Samples to prepare cDNA from
• Target vector and information
Further details on the project may be requested directly by the expert. You will be able to exchange directly with the experts after filling in the form below.

Delivrable(s)

Library in 96 or 384 plates well plates, filters or glycerol stock
Expected CFU number of 5.10^5 with an average size >1kb
Random sequencings included
Study following the specifications validated with the Expert

Your project

Please answer all questions below to specify your requirements

Number of samples from which a cDNA library needs to be prepared:

Construction type:

Directional cloning:

Library complexicity verification by random DNA sequencing: