Cell & tissue imaging
The "fluorescence microscope" refers to any microscope that uses fluorescence to generate an image. In this case, a simple set up is using epifluorescence to generate images, with a combination of filters and dichroic mirror. Generated picture typically have a low signal-to-noise ratio, and thus epifluorescence microscopy is the method of choice for most microscopy analysis in life sciences. Specific needs in terms of treatment, data collected, etc. can be specified to generate a full experimental observation.
• Samples (fixed cells or cells in culture)
• Cell growth conditions
• Plasmids and transfection conditions
• Requirements for time course
• Antibody for immunostaining and information regarding immunostaining protocol
• Requirements for quantitative experiment
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