Biomarkers Identification and Profiling

A biomarker is a measurable biological specificity related to normal or pathological activity in the body.


Biomarkers can be of several types: molecular biomarkers (proteins, peptides, genetic mutations, metabolites, small molecules) and biomarkers of imaging (tomography, MRI, etc.).

Many molecular markers have been identified, such as ACE (Carcino-Embryonic Antigen), BRCA1 and BRCA2 tumor markers, etc. or markers of Alzheimer's disease (t-Tau, p-Tau, Aβ1-42), and Parkinson's disease.

Other biomarkers are yet to be identified, and will serve as indicators in the development of therapeutic molecules (discovery), and eventually in the future, better detect the early signs of certain diseases to support them quickly (validation).

Cytokine analyses with ELISA Multiplex (Luminex and Quanterix SIMOA)   Mass spectrometry

Cytokine analyses with ELISA Multiplex (Luminex® and Quanterix SIMOA)

 

Mass spectrometry

 

 

The ELISA multiplex techniques are variants of the ELISA method, which allow the simultaneous analysis and assay of several proteins and biomarkers in the same sample.

 

These biomarkers are for example the cytokines and chemokines like interleukins and markers of inflammation. Other targets can be selected according to the pathologies or signaling pathways studied. Luminex and Quanterix SIMOA technologies (Single Molecule Arrays) are particularly sensitive.

Have look at our proteins, antibodies and antigens services 

 

A functional test will be applied on targets such as GPCR receptors (ie G proteins), cytokines (interleukins, chemokines, IFN), phosphorylated proteins (HER2, EGFR, AKT, etc.), and control points immune system (CD47, PDL1, etc.).

The study of metabolites and proteins by mass spectrometry has developed very rapidly over the past twenty years.

Today, mass spectrometry, and in particular the LC-MS / MS tandem spectrometry techniques, is particularly well positioned for the development of clinical tests. One of the recognized advantages of mass spectrometry is the ability to detect post-translational modifications (i.e. phosphorylation, ubiquitination, etc.).

Have look at our Analytical services 

Protein and DNA microarray  

Cellular and biochimical functional assays

Protein and DNA Microarray

 

Cellular & biochimical functional assays

Protein and DNA Microarray (or micro-chip) techniques are used to analyze the impact on cell signaling pathways of one or more elements or models.

 

Quantitative PCR methods (qPCR) are used to characterize and define the mode of action of biomarkers as well as to validate biomarkers previously identified in vitro.

 

Protein profiling by protein microarray is used in the discovery of surface markers and in large studies in Discovery. This can also be useful for determining a target of a lead (drug) as well as its mode of action.

Have a look at our sequencing, expression and edition services

 

Functional Cellular Assays can profile compounds by looking at their impact on defined targets.

 

FRET and HTRF techniques are typically used for test setup, and based on energy transfer between two fluorophores.

 

A functional test will be applied on targets such as GPCR receptors (ie G proteins), cytokines (interleukins, chemokines, IFN), phosphorylated proteins (HER2, EGFR, AKT, etc.), and control points immune system (CD47, PDL1, etc.).

Have a look at our cell culture, sorting and cell assays services

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