Monoclonal antibodies development

Service description

Monoclonal antibodies are antibodies made by an immune cell population coming from a unique clone. Steps to obtain monoclonal antibodies include: Immunization of an animal, like a mouse, collection of spleen cells, hybridization of cells with myeloma cells, culturing of the hybridomas, selection of positive antibody secreting clones, production in culture and testing. The main advantage of monoclonal antibodies is the reproducibility among batches of production.

Materials to provide

• Antigens in sufficient amount
• Details regarding the antigens
• Negative control if ELISA or Western blot confirmation
Further details on the project may be requested directly by the expert. You will be able to exchange directly with the experts after filling in the form below.

Delivrable(s)

ELISA data >1:128,000
Western blot data
Affinity purified antibody (quantity as required)
3 x vials of frozen cells for each requested clone (3 clones minimum)
QC report
Study following the specifications validated with the Expert

Your project

Please answer all questions below to specify your requirements

Amount of purified antibody for each clone:

ELISA confirmation of specificity (>1:128,000) (you need to provide negative controls):

Western blot confirmation of specificity (you need to provide negative controls):