EliSpot is a highly sensitive immunoassay based on the ELISA technique. It measures the frequency of cells that produce proteins and other individual effector molecules such as cytokines. This method can be used to calculate the frequencies of B and T cells. The ELISpot assay can provide more reliable quantitative and qualitative data compared to other methods. This technique of detction is used to evaluate the efficacy of vaccines and the immunogenicity of biological products.
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