Immunoprecipitation is a very sensitive and specific method to enrich a complex mixture with a protein of interest, or in the case of a co-immunoprecipitation, with proteins and molecules associated with the protein of interest. Different experimental outcomes can be important, such as quantity or specificity of protein immunoprecipitated, depletion of target, co-immunoprecipitation, coupling or radio-labelled protein expression assay (i.e. pulse chase exp).
Materials to provide
- Requirements for specific components in buffers
Further details on the project may be requested.
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- Extraction of proteins
- Purchase of antibodies
- Output as requested (IP in buffer, Gel, Western Blot, Co-IP test)
- Full detailed protocol
- Results analysis
- Study following the specifications validated with the Expert
Get started with your immunoprecipitation project
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