It may be necessary to know the mechanism by which an antibiotic exerts its antibacterial action.
A set of techniques can be used to identify the mechanism of action of antibiotics: a study of the permeabilizing action of the plasma membrane, microscopy and comparative observation of morphotypes caused by control and tested compounds, and radiolabelling techniques to measure the incorporation of precursors in the presence of control antibiotics.
Materials to provide
Compounds to test in sufficient amounts.
Further details on the project may be requested.
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Typical deliverables
Set up the assay.
Mechanism of action as determined using the selected assays.
Results analysis.
Study following the specifications validated with the Expert.
Get started with your antibacterial MOA identification project
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