mRNA sequencing uses Next-Generation Sequencing methods to look at the coding RNA in a given sample.
In order to discard the non-coding RNAs, two strategies can be adopted: either the hybridization of poly(A) tails using poly(T) tails, or the depletion of non-coding RNA by removing oligomers complementary to rRNA.
Materials to provide
Samples.
List of targets.
Proof of sample quality or integrity (Bioanalyzer traces or eq.).
Further details on the project may be requested.
You will be able to exchange directly with the experts after filling in the form below.
Typical deliverables
Extraction.
Library preparation.
Sequencing of the samples with the requested number of reads.
Paired-end read.
Quantification analysis.
Download the link or a hard drive with raw data and the post-experiment analysis.
Study following the specifications validated with the Expert.
Get started with your mRNA sequencing project
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