Plasmid expression vector cloning

43.1 - VecteurClonage

Service description

Molecular cloning is a process that allows the construction of recombinant DNA for the purpose of expressing genes in a given organism. The steps of cloning are typically: obtaining a source containing the DNA fragment of interest, amplification by PCR, insertion into a DNA vector. The set is inserted into a primary host for selection and replication (i.e. E. coli), and can then be purified to be introduced into a secondary host.

Materials to provide

  • Source of the gene and information
  • Primers and their sequences
  • Empty plasmid and its map

    Further details on the project may be requested.

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Typical deliverables

  • Bacterial clone on paper, glycerol or agar
  • Purified DNA
  • Sequencing of the sequence of interest
  • Report
  • Study following the specifications validated with the Expert

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