Plasmid expression vector cloning

43.1 - VecteurClonage

Service description

Molecular cloning is a process that allows the construction of recombinant DNA for the purpose of expressing genes in a given organism. The steps of cloning are typically: obtaining a source containing the DNA fragment of interest, amplification by PCR, insertion into a DNA vector. The set is inserted into a primary host for selection and replication (i.e. E. coli), and can then be purified to be introduced into a secondary host.

Materials to provide

  • Source of the gene and information
  • Primers and their sequences
  • Empty plasmid and its map

    Further details on the project may be requested.

You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Bacterial clone on paper, glycerol or agar
  • Purified DNA
  • Sequencing of the sequence of interest
  • Report
  • Study following the specifications validated with the Expert

Get started with your plasmid expression vector cloning

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