QPCR using multi probes

Genomics Genes expression QPCR using multi probes

Service description

Quantitative PCR (qPCR) with probes is a powerful technique that combines the amplification of a specific DNA fragment using PCR with the highly-sensitive detection of fluorescence in the sample using specific probes. In this case, multiple probes are used during the same amplification. Using different fluorophores will allow for the quantification of different amplifications.

Materials to provide

• Samples in sufficient amount
• Primers and probes, or accession number of the gene of interest
• Information regarding requested amplifications
• Description of the samples and QC data
• Description of the quantitation source, reference gene or threshold
Further details on the project may be requested directly by the expert. You will be able to exchange directly with the experts after filling in the form below.

Delivrable(s)

  • Extracts
  • RT-PCR
  • QC of extracts
  • NTC or NAC controls
  • Ordering primers and probes
  • qPCR experiment in triplicates or more
  • Endogenous control
  • Relative quantification
  • Data analysis
  • Study following the specifications validated with the Expert

Your project

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