cDNA library construction

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Service description

  • The construction of a cDNA library is a set of techniques that allows for the cloning of expressed genes in a given cell population into an identical vector.
  • It starts with the extraction of mRNA from a cell population, followed by a Reverse transcriptase PCR and an insertion of amplified sequences into a vector.
  • The vector will determine the subsequent usage of the library, i.e. standard library, subtractive, full-length, or Yeast 2-hybrid.

Materials to provide

  • Samples to prepare cDNA.
  • Target vector and information.

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Library in 96 or 384 plates well plates, filters, or glycerol stock.
  • CFU number.
  • Random sequencings included.
  • Study following the specifications validated with the Expert.
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Get started with your cDNA library construction

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