The construction of a cDNA library is a set of techniques that allows for the cloning of expressed genes in a given cell population into an identical vector.
It starts with the extraction of mRNA from a cell population, followed by a Reverse transcriptase PCR and an insertion of amplified sequences into a vector.
The vector will determine the subsequent usage of the library, i.e. standard library, subtractive, full-length, or Yeast 2-hybrid.
Materials to provide
Samples to prepare cDNA.
Target vector and information.
Further details on the project may be requested.
You will be able to exchange directly with the experts after filling in the form below.
Typical deliverables
Library in 96 or 384 plates well plates, filters, or glycerol stock.
CFU number.
Random sequencings included.
Study following the specifications validated with the Expert.
Get started with your cDNA library construction
Please answer the fields below to be contacted by our team of scientists
