cDNA library construction

49.1 - Banque-ADNc

Service description

The construction of a cDNA library is a set of techniques that allows for the cloning of expressed genes in a given cell population into an identical vector. It starts with the extration of mRNA from a cell population, followed by a Reverse transcriptase PCR and an insertion of amplified sequences into a vector. The vector will determine the subsequent usage of the library, i.e. standard library, substractive, full length or Yeast 2-hybrid.

Materials to provide

  • Samples to prepare cDNA from
  • Target vector and information

    Further details on the project may be requested.

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Typical deliverables

  • Library in 96 or 384 plates well plates, filters, or glycerol stock
  • CFU number
  • Random sequencings included
  • Study following the specifications validated with the Expert

Get started with your cDNA library construction

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