Next-Gen sequencing can generate a lot of data that might not be useful for every project.
To focus on the specific transcripts that matter for a given project, it is possible to create libraries only after the enrichment or amplification of a set of transcripts.
This is particularly useful in the case of difficult samples (such as paraffin-embedded tissue) or with low input of RNA (10 ng of total RNA), if the set of focus transcripts is identified.
Materials to provide
Samples.
List of targets.
Proof of samples quality or integrity (Bioanalyzer traces or eq.).
Further details on the project may be requested.
You will be able to exchange directly with the experts after filling in the form below.
Typical deliverables
Extraction.
Library preparation.
Depletion.
Sequencing of the samples with number of reads.
Paired-end reads.
Download link or a hard drive with raw data and the post-experiment analysis.
Study following the specifications validated with the Expert.
Get started with your RNA sequencing project
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