Digital PCR (dPCR or ddPCR) is a technique that separates the reaction of amplification of a specific DNA target by PCR into a large number of events.
Each event is measured for positive/negative amplification, and the number of positive amplifications will provide the absolute quantification of the initial specific DNA target in each sample using statistical laws.
Materials to provide
Samples in sufficient amounts.
Primers and probes, or accession number of the gene of interest.
Description of the samples and QC data.
Further details on the project may be requested.
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QC of extracts.
NTC or NAC controls.
Designing and ordering probes & primers.
dPCR experiment in replicates.
Study following the specifications validated with the Expert.
Get started with your Digital PCR project
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