Induced pluripotent cell line development

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Service description

  • The induced pluripotent stem cell lines (IPS or IPSC) are stem lines generated from already differentiated adult cells: they can multiply indefinitely, and be redifferentiated into any cell type of the human body.
  • The generation of IPSC begins with a culture phase of the donor cells, which are then epigenetically reprogrammed by the forced expression of pluripotency genes.
  • The vectors used for the introduction of the pluripotency genes may be of viral type (with a genetic modification of the donor cell), or of episomal, mRNA or Sendai type (in this case, the original cell is not genetically modified).
  • IPSCs are then selected and amplified by different methods.

Materials to provide

  • Primary cells/cell lines.
  • Culture conditions.

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Pluripotent cell lines and growth conditions.
  • Flow cytometry characterization.
  • Teratoma formation analysis.
  • Karyotyping.
  • Study following the specifications validated with the Expert.
Capture d’écran 2020-10-16 à 10.57.43

Get started with your IPS development project

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