Mammalian cell lines - Genome edition by CRISPR/Cas9

Gene editing 300

Service description

  • CRISPR/Cas9 is a powerful tool for genome editing. Using a specific vector, cell lines to be modified are transfected or infected by viral systems for DNA delivery.
  • Edited cell lines are selected, and the modifications of the genome are sequenced for verification.
  • Monoallelic vs biallelic edition can be chosen, as well as an optional removal of the selection cassette after integration.

Materials to provide

  • Accession number of genes of interest.
  • Guide vector prep and information.
  • Antibodies and conditions.

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Edited clones (number of clones defined by requester) in a frozen vial (3 x 1ml).
  • PCR verification of the gene edition.
  • Data for sorting enrichment.
  • Monoallelic verification.
  • Western blot verification.
  • Removal of selection marker.
  • Study following the specifications validated with the Expert.
Capture d’écran 2020-10-16 à 10.57.43

Get started with your CRISPR/Cas9 mammalian cell line genome editing project

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