Microscale thermophoresis

9 - Protein purification

Service description

Microscale thermophoresis (MST) is a technology for the biophysical analysis of interactions between biomolecules. This technique is based on the detection of a temperature-induced change in fluorescence of a target as a function of the concentration of a non-fluorescent ligand. MST is used to measure interactions in real-time, without partner immobilization, and in all types of complex biological buffers or liquids. Microscale thermophoresis can, with low sample quantities, provide information on affinity constants for a range of affinity between nM and mM, with high accuracy and affinity.

Materials to provide

  • Samples

    Further details on the project may be requested.

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Typical deliverables

  • Study following the specifications validated with the Expert

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