Monoclonal antibodies development
Monoclonal antibodies are antibodies made by an immune cell population coming from a unique clone. Steps to obtain monoclonal antibodies include: Immunization of an animal, like a mouse, collection of spleen cells, hybridization of cells with myeloma cells, culturing of the hybridomas, selection of positive antibody secreting clones, production in culture and testing. The main advantage of monoclonal antibodies is the reproducibility among batches of production.
Materials to provide
- Antigens in sufficient amount
- Details regarding the antigens
- Negative controls
Further details on the project may be requested.
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- ELISA data >1:128,000
- Western blot data
- Affinity purified antibody (quantity as required)
- QC report
- Study following the specifications validated with the Expert
Get started with your monoclonal antibody development
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