Monoclonal antibody sequencingis the first step to antibody engineering: optimization and recombinant expression.
This experiment allows for a fast, targeted sequencing of the DNA sequence expressing the antibody. In this case, the hybridoma cell line has been lost, and if the antibody is available in sufficient amounts, it is possible to identify the antibody sequence by mass spectrometry.
Materials to provide
Hybridoma expressing the antibodies to a sequence.
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DNA sequences of the coding region of the antibodies.
Peptidic sequences of the antibodies.
Sequencing of heavy and light chains.
Study following the specifications validated with the Expert.
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