Organelles purification - Peroxisomes
Peroxisomes are organelles found in all eukaryotic cells. They are involved in the catabolism of very long fatty acid chains, D-amino acids, polyamines, and in the synthesis of plasmalogens. Two levels of purification can be achieved: crude peroxisome fraction (CPF) purification, and highly purified peroxisomes.
Materials to provide
- Cells (if applicable)
Further details on the project may be requested.
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- Cell culture
- Peroxisomes isolation according to the amount provided
- Estimation of yield and purity
- Downstream extractions
- Study following the specifications validated with the Expert
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