Plasmid expression vector subcloning

Cloning 300

Service description

  • Sub-cloning corresponds to molecular cloning in which the gene of interest is integral to a fragment that can be simply cut and pasted into a new plasmid vector.
  • This process is an alternative to a DNA amplification of the gene prior to its insertion in a vector DNA.

Materials to provide

  • Sequence inserted in the origin plasmid.
  • Map of the plasmids.
  • Target plasmid.

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Bacterial clone on paper, glycerol, or agar.
  • Prep of purified DNA.
  • Sequencing of the insert.
  • Map of the plasmid with cloning strategy.
  • Study following the specifications validated with the Expert.
Capture d’écran 2020-10-16 à 10.57.43

Get started with your plasmid subcloning

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