Sub-cloning corresponds to molecular cloning in which the gene of interest is integral to a fragment that can be simply cut and pasted into a new plasmid vector.
This process is an alternative to a DNA amplification of the gene prior to its insertion in a vector DNA.
Materials to provide
Sequence inserted in the origin plasmid.
Map of the plasmids.
Target plasmid.
Further details on the project may be requested.
You will be able to exchange directly with the experts after filling in the form below.
Typical deliverables
Bacterial clone on paper, glycerol, or agar.
Prep of purified DNA.
Sequencing of the insert.
Map of the plasmid with cloning strategy.
Study following the specifications validated with the Expert.
Get started with your plasmid subcloning
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