Plasmid expression vector subcloning
Sub-cloning corresponds to a molecular cloning in which the gene of interest is integral to a fragment that can be simply cut and paste into a new plasmid vector. This process is an alternative to a DNA amplification of the gene prior to its insertion in a vector DNA.
Materials to provide
- Sequence inserted in the origin plasmid
- Map of the plasmids
- Target plasmid
Further details on the project may be requested.
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- Bacterial clone on paper, glycerol or agar
- Prep of purified DNA
- Sequencing of the insert
- Map of the plasmid with cloning strategy
- Study following the specifications validated with the Expert
Get started with your plasmid subcloning
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