Quantitative PCR (qPCR) with dye is a powerful technique that combines the amplification of a specific DNA fragment using PCR with the highly-sensitive detection of fluorescence in the sample using specific dyes.
The more amplification of DNA in the tube, the more dye will bind the double-stranded DNA and become fluorescent, thus allowing for a quantitative determination of initial specific DNA in the sample.
Materials to provide
Samples in sufficient amounts.
Primers, or accession number of the gene of interest.
Description of the samples and QC data.
Further details on the project may be requested.
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Typical deliverables
Extracts.
RT-PCR.
QC of extracts.
NTC or NAC controls.
qPCR experiment in replicates.
Controls.
Melting curve.
Relative quantification.
Data analysis.
Study following the specifications validated with the Expert.
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