Gene expression analysis by qPCR using dyes


Service description

  • Quantitative PCR (qPCR) with dye is a powerful technique that combines the amplification of a specific DNA fragment using PCR with the highly-sensitive detection of fluorescence in the sample using specific dyes.
  • The more amplification of DNA in the tube, the more dye will bind the double-stranded DNA and become fluorescent, thus allowing for a quantitative determination of initial specific DNA in the sample.

Materials to provide

  • Samples in sufficient amounts.
  • Primers, or accession number of the gene of interest.
  • Description of the samples and QC data.

  • Further details on the project may be requested.
  • You will be able to exchange directly with the experts after filling in the form below.

Typical deliverables

  • Extracts.
  • RT-PCR.
  • QC of extracts.
  • NTC or NAC controls.
  • qPCR experiment in replicates.
  • Controls.
  • Melting curve.
  • Relative quantification.
  • Data analysis.
  • Study following the specifications validated with the Expert.
Capture d’écran 2020-10-16 à 10.57.43

Get started with your

Please answer the fields below to be contacted by our team of scientists

favicon large un peu plus-1