Quantitative mass spectrometry analysis
Mass Spectrometry is an analytical chemistry technique that allows the quantification of proteins in a sample. Quantification can be absolute, using internal isotopic standards for targeted analyses only, or relative, with isotopic labelling such as SILAC, TMT or iTRAQ, or without labelling using the label-free technique. The services currently available offer relative quantification with label-free or labelled techniques. The performances of these two types of methods are equivalent. The SILAC approach is more robust because it limits experimental variability. It should be noted that it applies to cultured cells only and requires a large number of passages to ensure a high rate of isotope incorporation into proteins.
Materials to provide
- Description of samples
- Specific requirements description (if immunoprecipitation or chromatography enrichment)
Further details on the project may be requested.
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- Treatment of the samples
- Digestion (i.e. trypsin digestion) of the samples
- Peptide preparation and LC MS/MS read
- Quantitative and qualitative data analysis
- Post-translational modifications analysis
- Report file and advanced analysis
- Study following the specifications validated with the Expert
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